Rishi Ram Adhikari, M.Sc.

Kontakt

Universität Potsdam,
Institut für Erd- und Umweltwissenschaften

Rishi Ram Adhikari, M.Sc.
Haus 27, Raum 2.36

Karl-Liebknecht-Str. 24-25
14476 Potsdam-Golm

E-Mail:
adhikari@geo.uni-potsdam.de
Telefon:
+49 331 977 5858
Fax:
+49 331 977 5700
 Rishi Ram Adhikari , M.Sc.

 

  • Biografie
  • Forschung
  • Publikationen

Biografie

2008 onwards
PhD student, Institute of Earth and Environmental Sciences, University of Potsdam
2006
MSc Water Sciences, University of Duisburg-Essen, Germany
2002
BSc Chemistry, Tribhuvan University, Nepal

Forschung

Project: H2ase

An analytical technique that could quantify total microbial activity in sediments with low biomass and/or metabolic activity would greatly improve our understanding of subsurface life. So far, only specific processes (e.g. sulfate reduction, anaerobic oxidation of methane, methanogenesis) can be measured with sufficient sensitivity, there is no “catch all” measurement that provides a measure of total metabolic activity in subsurface environments. Assays of ubiquitous enzymes appear to be the most promising approach, however, most available assays lack the necessary sensitivity for application in subsurface sediments. A new radio-labeled enzymatic assay, targeting the enzyme family of hydrogenases (H2ases) has the potential to be the best subsurface microbial biomass activity quantification technique as it is highly sensitive and has already been proven to work in subsurface sediments (Nunoura et al., 2009, Schink et al., 1983, Soffientino et al., 2006).

Working Group

Publikationen

Røy, H., Kallmeyer, J., Adhikari, R.R., Pockalny, R., Jørgensen, B.B., D’Hondt, S., 2012. Aerobic Microbial Respiration in 86-Million-Year-Old Deep-Sea Red Clay. Science 336, 6083, 922-925. [PDF]

Adhikari, R.R., Kallmeyer, J., 2010. Detection and quantification of microbial activity in the subsurface. Chemie der Erde - Geochemistry 70, 135. [PDF]

Abstracts/Posters:

Adhikari, R.R., Nickel, J., Kallmeyer, J.,2012. Quantification of microbial activity in subsurface environments using a hydrogenase enzyme assay. Geophysical Research Abstracts Vol. 14, EGU2012-7959, EGU General Assembly 2012 Vienna. [PDF]

Adhikari, R.R., Kallmeyer, J., 2011. Hydrogenase enzyme assay for the quantification of microbial activity in subsurface environments. Mineralogical Magazine, Volume 75, Number 3, 408, Goldschmidt Conference Abstracts 2011 Prague. [Talk]

Røy, H., Kallmeyer, J., Adhikari, R.R., Gribsholt, B., Jørgensen, B.B., D’Hondt, S., 2011. Aerobic microbial respiration in ancient oxic sediments below the Subtropical Gyres. Geophysical Research Abstracts Vol. 13, EGU2011-8714, EGU General Assembly 2011 Vienna. [PDF]

Adhikari, R.R., Kallmeyer J., 2011. Hydrogenase enzyme assay: a promising technique for the quantification of microbial activity in subsurface environment. IODP/ICDP Workshop on Geological Carbon Capture & Storage 2011 Oman. [Poster]

Adhikari, R.R., Kallmeyer J., 2010. Hydrogenase enzyme assay: a promising technique for the quantification of microbial activity in subsurface environment. International GeoEn Meeting, Potsdam. [Poster]

Kallmeyer, J., Pockalny, R.A., D’Hondt, S.L., Adhikari, R.R., 2009. A New Estimate of Total Microbial Subseafloor Biomass, Eos Trans. AGU, 90(52), Fall Meet. Suppl., Abstract B23C-0381, California. [PDF]

Adhikari, R.R., 2009. Hydrogenase, a new tool for life detection and quantification of microbial activity. GeoEn Workshop „Geomikrobiologie”, GFZ German Research Centre for Geosciences, Potsdam. [Talk]

Biografie

2008 onwards
PhD student, Institute of Earth and Environmental Sciences, University of Potsdam
2006
MSc Water Sciences, University of Duisburg-Essen, Germany
2002
BSc Chemistry, Tribhuvan University, Nepal

Forschung

Project: H2ase

An analytical technique that could quantify total microbial activity in sediments with low biomass and/or metabolic activity would greatly improve our understanding of subsurface life. So far, only specific processes (e.g. sulfate reduction, anaerobic oxidation of methane, methanogenesis) can be measured with sufficient sensitivity, there is no “catch all” measurement that provides a measure of total metabolic activity in subsurface environments. Assays of ubiquitous enzymes appear to be the most promising approach, however, most available assays lack the necessary sensitivity for application in subsurface sediments. A new radio-labeled enzymatic assay, targeting the enzyme family of hydrogenases (H2ases) has the potential to be the best subsurface microbial biomass activity quantification technique as it is highly sensitive and has already been proven to work in subsurface sediments (Nunoura et al., 2009, Schink et al., 1983, Soffientino et al., 2006).

Working Group

Publikationen

Røy, H., Kallmeyer, J., Adhikari, R.R., Pockalny, R., Jørgensen, B.B., D’Hondt, S., 2012. Aerobic Microbial Respiration in 86-Million-Year-Old Deep-Sea Red Clay. Science 336, 6083, 922-925. [PDF]

Adhikari, R.R., Kallmeyer, J., 2010. Detection and quantification of microbial activity in the subsurface. Chemie der Erde - Geochemistry 70, 135. [PDF]

Abstracts/Posters:

Adhikari, R.R., Nickel, J., Kallmeyer, J.,2012. Quantification of microbial activity in subsurface environments using a hydrogenase enzyme assay. Geophysical Research Abstracts Vol. 14, EGU2012-7959, EGU General Assembly 2012 Vienna. [PDF]

Adhikari, R.R., Kallmeyer, J., 2011. Hydrogenase enzyme assay for the quantification of microbial activity in subsurface environments. Mineralogical Magazine, Volume 75, Number 3, 408, Goldschmidt Conference Abstracts 2011 Prague. [Talk]

Røy, H., Kallmeyer, J., Adhikari, R.R., Gribsholt, B., Jørgensen, B.B., D’Hondt, S., 2011. Aerobic microbial respiration in ancient oxic sediments below the Subtropical Gyres. Geophysical Research Abstracts Vol. 13, EGU2011-8714, EGU General Assembly 2011 Vienna. [PDF]

Adhikari, R.R., Kallmeyer J., 2011. Hydrogenase enzyme assay: a promising technique for the quantification of microbial activity in subsurface environment. IODP/ICDP Workshop on Geological Carbon Capture & Storage 2011 Oman. [Poster]

Adhikari, R.R., Kallmeyer J., 2010. Hydrogenase enzyme assay: a promising technique for the quantification of microbial activity in subsurface environment. International GeoEn Meeting, Potsdam. [Poster]

Kallmeyer, J., Pockalny, R.A., D’Hondt, S.L., Adhikari, R.R., 2009. A New Estimate of Total Microbial Subseafloor Biomass, Eos Trans. AGU, 90(52), Fall Meet. Suppl., Abstract B23C-0381, California. [PDF]

Adhikari, R.R., 2009. Hydrogenase, a new tool for life detection and quantification of microbial activity. GeoEn Workshop „Geomikrobiologie”, GFZ German Research Centre for Geosciences, Potsdam. [Talk]